

Waluigi's cap is still an unlockable hat for Mii Fighters. He cannot be summoned on the 3D Land, Midgar, Mute City SNES, Norfair, Spirit Train, and Suzaku Castle stages. Once again, Waluigi is an Assist Trophy, and defeating him rewards a point. In both versions, Waluigi's cap is an unlockable hat for Mii Fighters and a trophy can be unlocked by achieving a score of 85 or more in Rival Smash. He behaves the same as before, although he can be defeated if he takes enough damage. Waluigi appears once more as an Assist Trophy.

The second is based on Super Mario Strikers, and increases slash attack power for all Mario fighters. Additionally, a random collectable trophy can be obtained and two stickers are based on Waluigi the first is based on Mario Superstar Baseball and increases indirect attack power for all Mario fighters. When summoned, he will run to opponents and stomps them into the ground multiple times before swatting them away with his tennis racquet. Waluigi appears as a trophy, unlockable through the Lottery once at least 250 other trophies have been obtained. He also does the Moonwalk while doing a Trick Shot. Much like the Mario Brothers and Wario, he is this time in a tennis outfit: a purple polo, a tennis cap, black shorts and tennis shoes that are pointy and resemble his normal shoes.

His Special Shot seems to also have rose petals as its visual effect. His Special Shot, Showtime, involves him showing his theatrical side, which looks a lot like the animation for him getting an Eagle or better in Mario Golf World Tour He snaps his finger, gets a rose in his mouth, poses and spins around while screaming "WAAAAAAAAAAAAA" and levitates upwards. As a result, Waluigi players usually spam his Flat Shot. He has great reach and good shot power, but is rather slow and his shots don't curve very well. He used to be extremely overpowered, but is now a lot more balanced. In the game itself, Waluigi is a Defensive character much like he usually is. When they enter the stadium, they receive a dose of booing for their usual behavior in sports, such as foul play and bad sportsmanship.

Instead, however, they do a Doubles round against Princess Peach and Princess Daisy while Mario takes on the possessed Luigi. They do not do much in the story at first, but at the end of the story, they steal two of the Power Stones used to either empower or defeat Lucien right from under Mario's nose and challenge him to a duel in Marina Stadium. As this happens, the same fate is bestowed upon Waluigi and Wario. Luigi ends up taking Lucien instead, however, and becomes possessed by it, transforming into a mummified, evil version of himself. However, rather than themselves, Lucien forces them to give it to Mario so it can use his body to take over the world, perhaps enforcing the idea that Waluigi (and Wario) are seen as incompetent by about everyone. It is stated that they were easy to manipulate because of their greed. In the story, both him and Wario are under the control of Lucien, an ancient evil tennis racket. In Mario Tennis Aces, Waluigi plays the role as a minor antagonist in its Adventure mode. He also joined the Olympic Games and the Olympic Winter Games. Waluigi is a playable character in most of the Mario-sport games. If he wins in Mario Party 8, he holds out a long red rose, showing that he either has a sweet side or is simply being vain. In the movie, it is stated that the Wario Bros. Some would think that these two are pure evil, but the truth is that they are just bumbling crooks. Luigi is the positive spinoff of Mario and Wario is the negative spinoff of Mario, so Waluigi is not just a spinoff of Mario's design but also one for Wario because he needs a doppelganger brother of his own to even out Luigi. If this is true, then the term of "brother" in the other games just means friend/close relative. There is a theory that Wario and Waluigi (The Wario Bros.) are not Mario's brothers since in Mario Tennis, they sent a letter calling them cousins. He never truly appears as a villain in Mario games, with the exception of Dance Dance Revolution: Mario Mix.
